In this Imaging Optical Design Technical Group webinar, Fan Wang from the University of Technology Sydney will report on a strategy for applying upconversion nanoparticle as NIR imaging probe to mitigate absorption, whilst increase the imaging resolution by its nonlinear photo-response process, developing in-depth super-resolution microscopy methods. The talk will present a method that takes the advantage of “nondiffractive” Bessel beam, further employs the photon-saturation of the NIR emission from UCNPs, so that enabling super-resolution mapping of single nanoparticles located 55 µm inside a spheroid, with a resolution of 98 nm, without adaptive optics compensation. Wang further applies the photon-conversion of UCNPs for a high efficient NIR nonlinear structured illumination microscopy (NIR-NSIM) for rapid in-depth super-resolution imaging. With 10 kW/cm2 continuous wave (CW) excitation, NIR-NSIM achieves a resolution of 130 nm, 1/7th of the excitation wavelength, and a frame rate of 1 fps, through 50 µm biological tissues.

In the webinar, Wang will also report that doping rare-earth ions into low refractive index nanocrystal can significantly increase its trap stiffness for optical trapping. Wang demonstrates the steady trapping of a rare-earth ions doped nanoparticles with low refractive index 1.46, being doped with 20% of Ytterbium ions (Yb3+). For a particle with an effective diameter of 22 nm, they achieved a high trap stiffness of 0.08 pN/μm/mW, more than 22 times higher than the simulated undoped particle.

What You Will Learn:

• Upconversion nanoparticles
• Upconverting super-resolution microscopes include STED, GSD and SIM

Who Should Attend:

• Professors, students, researchers, engineers working on microscopy and optical tweezers

About the Presenter:  Fan Wang, University of Technology Sydney

Dr. Fan Wang is an Australian Research Council DECRA fellow, working on nanophotonics and biophotonics research. He has extensive expertise in optoelectronics, super-resolution imaging, and nanomaterial. Dr. Wang was awarded the UTS Chancellor’s Postdoctoral Research Fellowship (2019) to support his research in deep tissue super-resolution imaging. He has also been awarded the ARC DECRA fellow (2020) to conduct his research in biological laser cooling technology. Dr. Wang has published over 56 peer-reviewed journal articles with an h-index of 27 (Google Scholar) and over 2624 citations. His research articles are published in premier international journals, which has resulted in a very high average impact factor 10 over his publications.
 
Dr. Wang obtained his Ph.D. from the University of New South Wales in Australia in 2014. From 2013 to 2015, he worked as a Postdoctoral Research Fellow in Prof. Jagadish’s group at the Australian National University studying semiconductor nanowires. Since 2015, Dr. Wang joined the ARC center of excellence for nanoscale biophotonics at Macquarie University. From 2017, Dr. Wang joined Prof. Dayong Jin’s group in UTS to lead the biophotonics team. From 2020, Dr Wang joined the School of Electrical and Data Engineering as a lecturer to establish his nano-optoelectronics group.