Unveiling the Living Eye with Multiphoton Techniques


Unveiling the Living Eye with Multiphoton Techniques

Hosted By: Applications of Visual Science Technical Group

4 November 2020, 13:00 - 14:00

Download Presentation Slides

This OSA Applications of Visual Science Technical Group webinar will give a detailed overview of advanced multiphoton imaging, including Second Harmonic Generation (SHG), applied to the study of the eye with particular focus on the living human eye.

The webinar will consist of two talks:

Second Harmonic Generation Microscopy of the Cornea presented by Juan M. Bueno – Unlike commercial clinical devices, SHG microscopes are able to visualize non-stained collagen-based corneal tissues. SHG imaging represent a powerful tool to extract structural information and objectively distinguish healthy from pathological samples. This talk will show the last advances in SHG corneal imaging and its recent applications in living human eyes.

Multiphoton Imaging of the Living Retina presented by Christina Schwarz – Multiphoton microscopy has become a powerful tool for intravital imaging. This form of microscopy provides particular benefit when applied to the primate retina as it provides access to structural components and molecules involved in cellular metabolism and the visual cycle. This talk will discuss current applications, safety aspects and the future potential of multiphoton imaging of the retina.

What You Will Learn:

  • The concept of SHG and how the combination of two photons provides a “non-usual” imaging technique
  • How a transparent biological structure such as the corneal stroma can be visualized at a high resolution without the need for markers
  • How the living human cornea can be SHG imaged under safe conditions
  • How multiphoton imaging of the retina serves to study retinal structure and function
  • How imaging parameters can be chosen to avoid thermal, chemical and mechanical light damage to the eye

Who Should Attend:

  • Postgraduate students and researchers in vision sciences, biomedical optics, imaging, and ophthalmology
  • Clinicians and practitioners who would like to gain insight into how advanced optical imaging techniques are trying to make an impact in the study of the eye

About Our Presenters:

Juan M. Bueno, Universidad de Murcia

Juan M. Bueno holds a position as Full Professor of Optics (Department of Physics) at the University of Murcia (Murcia, Spain). He performs his research at the Center for Research in Optics and Nanophysics, leading the biomedical imaging team within the research group “Laboratorio de Optica”. His current research interests include nonlinear microscopy of ocular tissues and in particular the characterization of collagen-based structures through second harmonic generation techniques.



Christina Schwarz, University of Tübingen

Christina Schwarz is a Research Group Leader at the Institute for Ophthalmic Research at the University of Tübingen and an adjunct assistant professor at the University of Rochester. She earned her PhD in Vision Science from the University of Murcia in 2013. Since her postdoctoral period in Rochester (2014-2018), she has been pursuing the translation of two-photon ophthalmoscopy to human.