Computational Microscopy II (CTu4B)

Presider: Vidya Ganapati, Swarthmore College
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10:00 - 10:15
(UTC - 07:00)

Video-Rate Volumetric Imaging of Extended Structures with Engineered PSFs (CTu4B.1)
Presenter: Daniel Olesker, University of Glasgow

Imaging dynamic processes in 3D with high temporal resolution is a key challenge in fluorescence microscopy. By combining pupil-plane-engineering with a novel deconvolution algorithm we demonstrate a hybrid method that provides true snapshot volumetric imaging.

Authors:Daniel Olesker, University of Glasgow / Andrew Harvey, University of Glasgow / Jonathan Taylor, University of Glasgow


10:15 - 10:30
(UTC - 07:00)

Diffraction-limited Quantitative Phase Microscopy at peta-Hertz Rates (CTu4B.2)
Presenter: Gil Ilan Haham, Technion

We propose and explore theoretically and experimentally a single-shot multi-frame quantitative phase microscope, based on measurement of coded diffraction patterns concurrently. The proposed method also enables ultrahigh-speed imaging, paving the way to ultrahigh-speed, diffraction-limited microscopy.

Authors:Gil Ilan Haham, Technion / Or Peleg, Technion / Barry Loevsky, Technion / Pavel Sidorenko, Cornell University / Oren Cohen, Technion


10:30 - 10:45
(UTC - 07:00)

Local methods to improve cryo-electron microcopy maps (CTu4B.3)
Presenter: Javier Vargas, Universidad Complutense de Madrid

Cryo-electron microscopy (cryo-EM) has become a powerful technique for protein structure determination. However, there are still important issues to be addressed, such as, robust sharpening or local B-factors estimations. To address these issues, we present here new local methods to improve the analysis and interpretability of cryo-EM maps.

Authors:Satinder Kaur, McGill University / Ahmad Khalifa, McGill University / Swathi Adinarayana, McGill University / Ruben Sanchez-Garcia, University of Oxford / Daniel Wrapp, The University of Texas at Austin / Jason McLellan, The University of Texas at Austin / Khahn Huy Bui, McGill University / Javier Vargas, Universidad Complutense de Madrid


10:45 - 11:00
(UTC - 07:00)

Hong-Ou-Mandel Enabled 3D Quantum Microscopy (CTu4B.4)
Presenter: Ashley Lyons, University of Glasgow

We demonstrate micron-scale lateral and depth resolution imaging microscopy using photon bunching via HOM interference. 3D images are obtained without scanning using a SPAD camera and depth information is retrieved from two-photon coincidence events.

Authors:Bienvenu Ndagano, University of Glasgow / Hugo Defienne, University of Glasgow / Ashley Lyons, University of Glasgow / Daniele Faccio, University of Glasgow


11:00 - 11:15
(UTC - 07:00)

Robustly Detecting Imaging Model Mismatches and Reconstruction Artifacts in Single-Molecule Localization Microscopy (CTu4B.5)
Presenter: Matthew Lew, Washington University in St. Louis

We demonstrate Wasserstein-induced flux (WIF) for measuring localization stability in localization microscopy. Without knowing the ground truth structure being imaged, WIF detects model mismatch within reconstruction algorithms and nanoscale imaging artifacts with high sensitivity.

Authors:Hesam Mazidi, Washington University in St. Louis / Tianben Ding, Washington University in St. Louis / Arye Nehorai, Washington University in St. Louis / Matthew Lew, Washington University in St. Louis


11:15 - 11:30
(UTC - 07:00)

Single-Shot Fluorescence Lifetime Imaging Microscopy Utilising a Time-Folding Optical Cavity (CTu4B.6)
Presenter: Vytautas Zickus, University of Glasgow

We report a new technique for fluorescence lifetime imaging microscopy, using a time-folded optical cavity and a gated camera, capable of retrieving arbitrarily-high-resolution lifetime maps from single-shot acquisitions. The technique enables lifetime imaging at video-rate.

Authors:Guillem Carles, University of Glasgow / Valentin Kapitany, University of Glasgow / Vytautas Zickus, University of Glasgow / Daniele Faccio, University of Glasgow


11:30 - 11:45
(UTC - 07:00)

Multispectral Fluorescence Lifetime Imaging with Single-Pixel Cameras and Data Fusion (CTu4B.7)
Presenter: Armin Lenz, Universitat Jaume I

We present a technique for multispectral fluorescence lifetime imaging with high spatial resolution by combining both single-pixel and data fusion imaging techniques. The resultant 4D hypercube can be used to identify fluorophore species.

Authors:Fernando Soldevila, ENS-Université PSL, CNRS, Sorbonne Université, Collège de France / Armin Lenz, Universitat Jaume I / Alberto Ghezzi, Politecnico di Milano / Andrea Farina, Consiglio Nazionale delle Ricerche / Cosimo d'Andrea, Politecnico di Milano / Enrique Tajahuerce, Universitat Jaume I


11:45 - 12:00
(UTC - 07:00)

Cross-correlation interferometric synthetic aperture microscopy (CC-ISAM) for Fourier-domain OCT (CTu4B.8)
Presenter: Arturo Canales, The Institute of Optics

CC-ISAM is a synthetic-phase modulation method in combination with an ISAM-based reconstruction algorithm that provides coherent noise-free, high-resolution, 3D imaging to data acquired by means of Fourier-domain optical coherence tomography (FD-OCT). Numerical results are presented.

Authors:Arturo Canales, The Institute of Optics / Scott Carney, The Institute of Optics