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12 April 2021 – 16 April 2021 OSA Virtual Event - Pacific Daylight Time (UTC - 07:00)

Sandrine Lévêque-Fort

Paris Saclay University

Alternative Strategies for 3D Single Molecule Localization Microscopy

I will present our recent work on 3D imaging in Single Molecule Localization Microscopy, where intrinsic properties of fluorescence emission can be directly used to enhance the resolution.

I will present our recent work on 3D imaging in Single Molecule Localization Microscopy, where intrinsic properties of fluorescence emission can be directly used to enhance the resolution.

About the Speaker

Sandrine Lévêque-Fort is a CNRS Researcher Director at the Institute of molecular science (ISMO) in Paris Saclay University. She obtained her PhD on the development of a new acousto-optic imaging approach for imaging through scattering media in the Optical Lab of ESPCI in Paris. She then became a postdoctoral fellow in the physics department of Imperial College, where she started to develop time resolved fluorescence microscopy but also structured illumination strategy. She joined the CNRS in 2001 to develop different strategies to improve spatial and temporal resolution for fluorescence microscopy, by implementing new configurations or used plasmonics substrates to engineered fluorescence emission. Since 2009, she has proposed various approaches to take advantage of supercritical angle fluorescence (SAF) emission as an alternative intrinsic tool given by the fluorophore itself to access axial information. In wide field microscopy this allows a dual depth imaging without any photon loss while preserving ideal sectioning for membrane imaging. In combination with super-resolution microscopy techniques (DONALD/DAISY), these new approaches permit to reveal quantitatively the 3D cellular nanoarchitecture. Since 2016, she has combined structured excitation with single molecule localization. By introducing a time signature within the localization process, this technique called ModLoc permits to retrieve the fluorophores’ information thanks to the phase of their modulated emission and benefits of an enhanced and uniform localization precision.
Sandrine Lévêque-Fort is a CNRS Researcher Director at the Institute of molecular science (ISMO) in Paris Saclay University. She obtained her PhD on the development of a new acousto-optic imaging...

Dan Oron

Weizmann Institute of Science

Quantum Enhanced Superresolution Confocal Microscopy

We show how the resolution of a standard confocal can be increased fourfold with a twofold axial resolution increase by harnessing the quantum phenomenon of fluorescence antibunching and by its classical analog of fluorescence intermittency.

We show how the resolution of a standard confocal can be increased fourfold with a twofold axial resolution increase by harnessing the quantum phenomenon of fluorescence antibunching and by its...

About the Speaker

Dan Oron earned a B.Sc. in mathematics and physics from the Hebrew university in 1994. He earned his M.Sc. degree in physics from Ben-Gurion University of the Negev in 1998 and received his Ph.D., also in physics, from the Weizmann Institute of Science in 2005, under the guidance of Prof. Yaron Silberberg. After conducting postgraduate research with Prof. Uri Banin at the Hebrew University for two years, he joined the staff of the Weizmann Institute in April 2007. He is currently a professor at the department of Molecular Chemistry and Materials Science at the Weizmann institute. His main research interests are at the interface between light and the nanoscale, studying both the interaction of light with nanostructured materials (mostly inorganic and hybrid semiconductor nanocrystals), optical superresolution methods harnessing both quantum and classical fluctuations in light emission and the optics of biological nanostructured materials.
Dan Oron earned a B.Sc. in mathematics and physics from the Hebrew university in 1994. He earned his M.Sc. degree in physics from Ben-Gurion University of the Negev in 1998 and received his Ph.D...

R. Clay Reid

The Allen Institute for Brain Science

Petascale Microscopy for Brain Mapping: Electron and Light Microscopic Approaches to Connectomics

The reconstruction of neural pathways and connections (connectomics) requires high-resolution microscopy over large volumes, thus requiring extremely large data sets. I’ll discuss approaches, from data collection through segmentation, for analyzing neural circuits at the petascale.

The reconstruction of neural pathways and connections (connectomics) requires high-resolution microscopy over large volumes, thus requiring extremely large data sets. I’ll discuss...

About the Speaker

Clay Reid is Senior Investigator at the Allen Institute for Brain Science, where he started a department in 2012 to study how information is encoded and processed in neural networks of the visual system. Prior to joining the Allen Institute, Reid was Professor of Neurobiology at Harvard Medical School. Throughout his career, he has used a combination of imaging and anatomical approaches to investigate how the structure of neural connections relates to the function of cortical circuits. He has helped to pioneer new methods for recording increasingly large ensembles of neurons to study sensory processing. In parallel, he has developed methods to analyze connections in these ensembles using large-scale anatomical reconstructions (connectomics) with serial-section electron microscopy.
Clay Reid is Senior Investigator at the Allen Institute for Brain Science, where he started a department in 2012 to study how information is encoded and processed in neural networks of the visual...