Microscopy, Histopathology and Analytics

Microscopy, Histopathology and Analytics

03 - 06 April 2018
The Diplomat Beach Resort, Hollywood, Florida United States

Example topics central to this meeting are novel forms of microscopies, computational modeling and algorithms, novel contrast agents, clinical analytics and translation to applications. Example applications areas are clinical diagnostics, surgical and non-surgical therapy guidance, prognostic procedures, high throughput drug screening and biomarker evaluation.


Topics

  1. Novel technology, devices, methods, models
    • Computational microscopy, including Fourier ptychography
    • Compressive and information-efficient sensing
    • Lens-free microscopy
    • Phase and birefringence microscopy
    • Nonlinear microscopy
    • Raman microscopy
    • UV microscopy
    • Multispectral microscopy
    • Global-health-related technologies (e.g., cell-phone-enabled low-resource-compatible pathology and diagnostics)
  2. Machine learning approaches and algorithms
    • Computational modeling
    • Diagnostic algorithms
    • Prognostic algorithms
    • Algorithms that combine optical microscopy with clinical and/or genetic data in patients
    • Machine learning, including neural networks and other configurations for diagnostic, prognostic, and high-dimensional data analysis
    • Image processing and analytics
  3. Enabling reagents and labeling technologies
    • Optical barcoding
    • Ultrabright, ultra-sharp optical labels
    • Rapid fresh-tissue labeling methods
    • novel contrast agents, reporters, probes, molecules
  4. Clinical analytics and microfluidics
    • Fluorescence correlation spectroscopy (FCS)
    • Optics in lab-on-a-chip, organ-on-a-chip, and microfluidic devices
    • Plate-reader technologies
    • FRAP, FLIM, and related methods
  5. Challenges in translation
    • From bench to market - scale up while lowering cost
    • Standardization of designs and processes
    • Design of validation studies
    • Understanding real world obstacles

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Speakers

  • Nathalie Agar, Harvard Medical SchoolUnited States 
    Molecular Imaging in Surgical Pathology and Drug Development
  • Gabriela Apiou-Sbirlea, Massachusetts General HospitalUnited States 
    Challenges, Opportunities and New Models to Enhance Translation of Fundamental Research to Patient Care
  • Rohit Bhargava, Univ of Illinois at Urbana-ChampaignUnited States 
    Infrared Spectroscopic Imaging for All-digital Molecular Histopathology
  • Jane Brock, Brigham & Womens HospitalUnited States 
    Making Breast Cancer Diagnoses in Low-Resource Settings
  • J. Quincy Brown, Tulane UniversityUnited States 
    Structured Light and Computational Topology for Next Generation Prostate Pathology
  • Brian Cunningham, Univ of Illinois at Urbana-ChampaignUnited States 
    Photonic Crystal Resonant Coupling to Nanoantennas and Applications for Digital Resolution Biosensing
  • David Dickensheets, Montana State UniversityUnited States 
    Toward Smaller, More Useable Clinical Confocal Microscopes with Advanced Scanning and Integrated Wide-Field Guidance
  • Marica Ericson, Sahlgrenska University HospitalSweden 
    Laser Scanning Microscopy Targeting Dermatology - Insights from Research and Translational Inertia
  • Reto Fiolka, UT SouthwesternUnited States 
    Quantitative imaging in 3D microenvironments using advanced light-sheet microscopes
  • Adam Glaser, University of Washington 
    Pathology-Optimized Open-Top Light-Sheet Microscopy
  • Bojana Gligorijevic, Temple UniversityUnited States 
    Real-Time Imaging of Invadopodia in Tumor Microenvironment Context
  • Laurie Goodman, Clearlight DiagnosticsUnited States 
    Providing CLARITY: 3D Imaging of Biomarkers in Tumor Samples
  • Attiya Haroon, Rutgers UniversityUnited States 
    Application of non-invasive diagnostic techniques for management of skin lesions in 21st century
  • Sheng-Lung Huang, National Taiwan UniversityTaiwan 
    Nucleus and Cytoplasm Segmentation using Full-Field Optical Coherence Tomography
  • Cecil Joseph, University of Massachusetts LowellUnited States 
    Investigating Multimodal Optical/Terahertz Imaging for Intraoperative margin delineation of Nonmelanoma Skin Cancers
  • DongKyun Kang, Massachusetts General HospitalUnited States 
    Spectrally encoded confocal microscopy for comprehensive and low-cost in vivo cellular imaging
  • Kivanc Kose, Memorial Sloan Kettering Cancer CenterUnited States 
    A Multiresolution Deep Learning Framework for Automated Annotation of Reflectance Confocal Microscopy Images
  • Aydogan Ozcan, University of California Los AngelesUnited States 
    Deep Learning Microscopy
  • Gabriel Popescu, Univ of Illinois at Urbana-ChampaignUnited States 
    SLIM tissue scanner for cancer diagnosis and prognosis in both stained and unstained tissues
  • Thomas Reiner, Stride Tool IncUnited States 
    Molecularly Targeted Imaging of Oral Cancer
  • Michael Shribak, Marine Biological LaboratoryUnited States 
    Quantitative Differential Interference Contrast Microscopy
  • Chi-Kuang Sun, National Taiwan UniversityTaiwan 
    Noninvasive Histopathological Imaging by Using Harmonic Generation Microscopy for Onsite Differential Diagnosis and Treatment Assessment
  • Eric Tkaczyk, Vanderbilt UniversityUnited States 
    Bringing Biophotonics to Dermatology Patients: Experiences of a New Cutaneous Imaging Clinic
  • Gracie Vargas, University of Texas at AustinUnited States 
    Exploring the combination of large area widefield fluorescence imaging with multiphoton microscopy in the detection of oral epithelial neoplasia
  • Alex Walsh, US Air Force Research LaboratoryUnited States 
    Imaging T Cell Metabolism for Activity and Heterogeneity Analysis
  • Benjamin Wilson, Intellectual VenturesUnited States 
    The Challenges and Prospects of Microscopy and Biophotonics in Low Resource Settings
  • Gang Yao, University of Missouri-ColumbiaUnited States 
    Nondestructive Polarimetric Imaging of Collagen Organization in Articular Cartilage
  • Haishan Zeng, BC Cancer Agency Research CentreCanada 
    Integrated Multimodality Microscopy and Confocal Raman Spectroscopy for In Vivo Skin Evaluation and Diagnosis
  • Andries Zijlstra, Vanderbilt UniversityUnited States 
    Integration of histocytometry and bioinformatics: Leveraging large scale bioimage processing to predict patient survival.

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Committee

  • Richard Levenson, University of California Davis, United States , Chair
  • Melissa Skala, University of Wisconsin-Madison, United States , Chair
  • Kevin Eliceiri, University of Wisconsin, United States , Program Chair
  • Milind Rajadhyaksha, Memorial Sloan Kettering Cancer Center, United States , Program Chair
  • James Castracane, SUNY Polytechnic Institute, United States
  • Andrew Cohen, University of Wisconsin-Milwaukee, United States
  • Xiaohu Gao, University of Washington, United States
  • Michael Giacomelli, Massachusetts Institute of Technology, United States
  • Jonathan Liu, University of Washington, United States
  • John Tomaszewski, State University of New York at Buffalo, United States
  • Benjamin Vakoc, Harvard Medical School, United States
  • Anna Yaroslavsky, Unoversity of Massacusetts, United States

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Plenary Session

Stephen A. Boppart

Beckman Institute for Advanced Science and technology, University of Illinois at Urbana-Champaign, USA

Stain-Free Slide-Free Multiphoton Histopathology of Carcinogenesis and Cancer

Multiphoton microscopy has emerged as a powerful tool for stain-free slide-free histopathology. We have recently demonstrated a fiber-based nonlinear optical microscope that achieves fast and simultaneous visualization of a variety of intrinsic molecular contrasts within live tissue including auto-fluorescence excited by two/three-photon processes and specially structured molecules by second/third harmonic generation. Results from human subjects with and without breast cancer, and a pre-clinical rat mammary tumor model, show that extracellular vesicles (exosomes and microvesicles) may serve as new biomarkers for breast cancer. Real-time videos also demonstrate the versatility of this imaging platform in tracking cellular events, including tumor cell migration and leukocyte activation in living rats with carcinogen-induced mammary tumors.  

Histopathology, whether with standard hematoxylin and eosin or special immunohistochemical stains, has been the gold-standard process for the diagnosis of disease.  However, due to the intensive time and labor required for the histochemical treatment of the tissue in traditional histopathology, great efforts have been devoted to using label-free optical imaging for the examination of intact biological specimens, even in vivo. By shifting to a new excitation wavelength, our fiber-based multimodal nonlinear optical microscope achieves fast and simultaneous visualization of the rich intrinsic molecular information within fresh human breast tissue.  

In addition to the near-real-time visualization of the authentic tumor microenvironment, quantitative analysis of these multi-dimensional datasets was performed in search for more selective clinical biomarkers. Preliminary analysis conducted on fresh human breast tissue obtained from healthy and cancer subjects showed that a significant portion of extracellular vesicles from the tumor micro- and macro-environment have unique optical signatures in comparison with those from healthy subjects, opening investigations of their physiological role in carcinogenesis and their diagnostic value for cancer.  Collectively, the potential exists for real-time stain-free in vivohistopathology, promoting imaging-based scientific research, and potentially revealing new image-based biomarkers for diseases such as cancer.  

About the Speaker

Professor Boppart heads the Biophotonics Imaging Laboratory at the Beckman Institute and is a full-time faculty member in the Bioimaging Science and Technology group. His home departments are Electrical and Computer Engineering and Bioengineering, with affiliations with the Department of Internal Medicine in the College of Medicine, the Micro and Nanotechnology Laboratory and the Institute for Genomic Biology. Professor Boppart received his PhD in Electrical and Medical Engineering from MIT in 1998 and his MD from Harvard Medical School in 2000. Currently he combines his optical imaging and biophotonics research and teaching with clinical research in novel medical technologies.

Eric R. Fossum

Thayer School of Engineering, Dartmouth University, USA

Quanta Image Sensor: Photon counting with high resolution and high frame rate

The Quanta Image Sensor will be introduced and recent progress presented from a 1Mpixel, 1000fps, back-illuminated room temperature stacked device. The prototype device features very low dark count rate and high quantum efficiency with further improvements anticipated in the future.

About the Speaker

Dr. Eric R. Fossum is best known for the invention of the CMOS image sensor “camera-on-a-chip” used in billions of cameras, from smart phones to web cameras to pill cameras and many other applications.  He has been a Professor with the Thayer School of Engineering since 2010.  He also serves as Dartmouth’s Associate Provost for Entrepreneurship and Technology Transfer. Fossum is a Queen Elizabeth Prize Laureate, the world’s largest engineering prize, a member of the National Academy of Engineering, a National Inventors Hall of Fame inductee, and a Fellow member of the IEEE and OSA.

Irene Georgakoudi

School of Engineering, Tufts University, USA

Unraveling the origins of endogenous optical metabolic changes

The ability to monitor subcellular functional and structural changes associated with metabolism is essential for understanding tissue development and disease progression. Metabolic perturbations or dysfunctions often play a critical role in numerous diseases, including cancer, obesity, cardiovascular and neurodegenerative disorders. Established methods to assess metabolic processes are either destructive or require the use of exogenous compounds. However, such approaches are limited in their ability to capture the highly dynamic and heterogeneous nature of metabolic responses.  In my plenary presentation, I will explain how a combined use of endogenous two-photon intensity and lifetime based fluorescence measurements enable ways to identify changes in specific metabolic pathways. These functional insights can improve understanding of disease development and drug effects.

About the Speaker
Irene Georgakoudi has been working on the use of lasers for therapeutic and diagnostic applications since her undergraduate years. She started as a physicist at Dartmouth College and continued her graduate studies in Biophysics at the University of Rochester. Her interests in spectroscopy and spectroscopic imaging using endogenous sources of contrast were founded during her postdoctoral years at the MIT Spectroscopy Lab. After working on the development of fluorescence-based in vivo flow cytometry while an Instructor at the Wellman Laboratories for Photomedicine at Massachusetts General Hospital/Harvard Medical School, she moved to Tufts in 2004. She is the author of several patents on the development and use of spectroscopy and imaging to characterize tissues or to detect specific populations of cells and has published numerous peer reviewed manuscripts, review articles and book chapters in these topics. She is the recipient of a Claflin Distinguished Scholar, an NSF Career, and an American Cancer Society Research Scholar award. She has served on the Board of Directors of the Optical Society of America, and is the Director of the Tufts Advanced Microscopic Imaging Center (TAMIC).

Nozomi Nishimura

Department of Biomedical Engineering, Cornell University, USA

Exploring natural behaviors of cells in the wild with in vivo multiphoton microscopy
Two-photon microscopy has become an important tool for experimental biology because it enables the dynamic imaging of cellular structures within a whole, living organism. Recent progress such as novel preparations, three-photon microscopy and third harmonic generation imaging reveal new phenomena in mouse models.
About the Speaker
Nozomi Nishimura majored in Physics at Harvard College where she worked with Prof. Eric Mazur on femtosecond laser ablation. In graduate school she became interested in neuroscience and worked with Prof. David Kleinfeld at University of California at San Diego. Although still in the Physics Department, her research focused on studying blood flow in the brain of rodents and developing laser-based models of small stroke. She went to Biomedical Engineering at Cornell in 2006 to do a postdoc with Prof. Chris Schaffer. She became an Assistant Professor in Biomedical Engineering in 2013. At Cornell, current research expands the use of in vivo imaging techniques to study a variety of disorders including Alzheimer's disease, cardiac disease and cancer metastasis. As a postdoc, she was awarded a L'Oreal USA Fellowship for Women in Science, the NIH Ruth L. Kirschstein NRSA Postdoctoral Fellowship, and the American Heart Association Postdoctoral Fellowship and while at UC San Diego, she received a National Science Foundation Graduate Research Fellowship.

Kandice Tanner

Laboratory of Cell Biology, Center for Cancer Research, National Institutes of Health, USA

Using Optical tweezers to probe the role of tissue biophysics in metastasis
Tumor latency and dormancy are obstacles in effective treatment of cancer. In the event of metastastic disease, emergence of a lesion can occur at varying intervals from diagnosis and in some cases following successful treatment of the primary tumor. Genetic factors that drive metastatic progression have been identified, such as those involved in cell adhesion, signaling, extravasation and metabolism.  Is there a difference in strategy to facilitate outgrowth? Why is there a difference in latency? One missing cue may be the role of tissue biophysics of the brain microenvironment on the infiltrated cells.  Here I discuss using optical tweezer based active microrheology to measure the mechanical cues that may influence disseminated tumor cells in different organ microenvironment. I further discuss in vitro and in vivo preclinical models such as 3D culture systems and zebrafish in efforts of providing novel targeted therapeutics.
About the Speaker
Kandice Tanner received her doctoral degree in Physics at the University of Illinois, Urbana-Champaign under Professor Enrico Gratton. She completed post-doctoral training at the University of California, Irvine specializing in dynamic imaging of thick tissues. She then became a Department of Defense Breast Cancer Post-doctoral fellow jointly at University of California, Berkeley and Lawrence Berkeley National Laboratory under Dr. Mina J. Bissell. Dr. Tanner joined the National Cancer Institute as a Stadtman Tenure-Track Investigator in July, 2012, where she integrates concepts from molecular biophysics and cell biology to learn how cells and tissues sense and respond to their physical microenvironment, and to thereby design therapeutics and cellular biotechnology. For her work, she has been awarded the 2013 National Cancer Institute Director’s Intramural Innovation Award, the 2015 NCI Leading Diversity award, 2016 Federal Technology Transfer Award, the 2016 Young Fluorescence Investigator award from the Biophysical Society and named as a Young Innovator in Cellular and Molecular Bioengineering, which highlight her scientific accomplishments and service to the greater intramural NIH and extramural scientific community. She also maintains strong connections with the extramural community through service as an editorial board member of Scientific Reports and as a review editor for Frontiers in Cell and Development Biology. She currently serves on the Membership Committee of the American Society of Cell Biology, the Minority Affairs Committee of the Biophysical Society and is a Member at large for the Division of Biological Physics of the American Physical Society.

Arjun G. Yodh

Department of Physics and Astronomy at the University of Pennsylvania, USA

Diffuse Optical Monitoring of Biomarkers in Brain and Breast
I will describe our progress applying diffuse optical monitoring techniques to measure hemodynamics, metabolism, and autoregulation in brain and breast tissues. The brain studies demonstrate potential for usage as a bedside treatment management tool in the neuro-ICU, especially for patients with traumatic brain injury and acute stroke; the breast studies demonstrate potential of optical cancer therapy monitoring, especially for patients undergoing neoadjuvant chemotherapy prior to surgery. 
About the Speaker
Arjun G. Yodh is the James M. Skinner Professor of Science in the Department of Physics and Astronomy at the University of Pennsylvania. At Penn, he is also Director of The Laboratory for Research on the Structure of Matter (LRSM) and its NSF-supported Materials Science and Engineering Center (MRSEC).  Yodh has published over 300 papers (>32,000 citations, h-index 96) about research that spans the fields of Biomedical OpticsCondensed Matter Physics, and Atomic, Molecular & Optical Sciences. His biomedical research is oriented towards diffuse optical imaging and monitoring of brain, breast, and muscle, and towards monitoring of hemodynamic biomarkers during therapy. His group made key contributions to the development of this field starting from its earliest stages, including identification of connections between traditional and diffuse optics, elucidation of diffuse optical resolution and contrast limits, experimental demonstration of frequency-domain diffuse optical tomography, imaging of exogenous contrast agents in human breast cancer based on both absorption contrast and fluorescence contrast, development of diffuse correlation spectroscopy for measurement of tissue blood flow, first all-optical measurements of tissue oxygen metabolism in human brain, technique validation of the optical methods in pre-clinical and clinical contexts against MRI, Xe-CT, ultrasound and other traditional techniques, and more. Yodh is a Fellow of the OSA, APS, AAAS, and AIMBE. 

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Special Events

NIRFAST Pre-Conference Training Workshop

Monday, 2 April, 08:00–18:00
The Diplomat Beach Resort, Meeting Room 314

The goal of this workshop is to provide hands-on instruction with NIRFAST for modeling light transport in tissue. The workshop will cover basic aspects of Nirfast and will include user driven examples on modeling light transport in tissues and performing tomographic image reconstruction. 

MCX'18 Pre-Conference Workshop

Monday, 2 April, 08:00–18:00
The Diplomat Beach Resort, Meeting Room 307

In this workshop, you will be provided with hands-on trainings on building fast and accurate Monte Carlo (MC) biophotonics simulations using open-source software - MCX (GPU accelerated MC simulator) and MMC (Mesh-based MC for accurate anatomical modeling). 
 

OSA Biomedical Optics Technical Groups Poster Award

Tuesday, 3 April - Thursday, 5 April
The Diplomat Beach Resort, Grand Ballroom East

OSA offers several technical groups focused on biomedical optics to provide you with a chance to connect with colleagues working in your area of specialization. As part of this congress, these technical groups are supporting an award for Best Poster Presentation for students and recent graduates.
 

Welcome Reception with Exhibitors

Tuesday, 3 April, 17:30–19:00
The Diplomat Beach Resort, Grand Ballroom East

Join your fellow attendees for the Congress Welcome Reception. Enjoy delectable fare while networking. One Welcome Reception ticket is included in the Full Technical Registration Fee. Guest tickets may be purchased for US $75.

Student & Early Career Professional Development & Networking Lunch and Learn

Wednesday, 4 April, 12:00–13:30
The Diplomat Beach Resort, Meeting Room 314
 
Join us for an interactive lunch and learn program focused on professional development within the Biophotonics field. This program will engage students and early career professionals with the key leaders in the field who will share their professional development journey and provide useful tips to those who attend. Lunch will be provided.

Hosted By:

 

Dinner Cruise on The Grand Floridian

Wednesday, 4 April, 18:00–20:00
The Diplomat Beach Resort, Diplomat Landing, Marina Dock

Attendee Ticket (Tickets are not included in registration fee): US $25
Guest Ticket: US $75

Cruise the Intracoastal waterway and enjoy dinner on a spectacular luxury yacht that offers four decks, a covered sky lounge and a spacious top deck for ocean views. Conference attendees may purchase extra tickets for their guests. Advance ticket purchase is required.

Cruise Schedule:
 
18:00–18:30 Boarding at the Diplomat Landing, Marina Dock
18:30 Cruise Departure
18:30–20:00 Cruise and Dinner Buffet
20:00 Return to Dock
21:00 Final Disembark
  

Workshop: Understanding Unconscious Bias

Thursday, 5 April, 12:00–13:30
The Diplomat Beach Resort, Atlantic Ballroom 1


Speaker: Sara Bendoraitis, American University, USA

Research demonstrates that we all have unconscious biases. These biases can result in best and brightest talent made to feel unwelcome, invisible, and not important to the success of the organization. This training will explore concepts and engage participants to better understand implicit bias, increase awareness and understanding the impact on organizational culture and identify ways to promote greater engagement with diversity and inclusion.

Hosted By:


 

Lost in Translation?: Clinical trial challenges across the Globe

Friday, 6 April, 12:00–13:30
The Diplomat Beach Resort, Grand Ballroom West


The potential for biophotonics technologies to have transformative clinical impact is clear. However, taking a great idea from the bench to the clinic, and then to market is a complex path to navigate. At this lunch event you will hear from a panel of experts who have taken on this challenge in order to deliver their technologies to those who need them. In particular, we will contrast the differences in design features and deployment strategies needed for technologies targeting markets such as the US and Europe, versus technologies for global health – an area well matched to the unique benefits of biophotonics. There will be ample time for questions, discussion and networking.
 

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