European Conferences on Biomedical Optics (ECBO)

12 - 16 maio 2013
Messe Munchen, Munich, Germany

Invited Speakers

2013 LASER World of PHOTONICS

Opening Plenary Talk on Nanoscopy with Focused Light
Stefan W. Hell, Max Planck Institute for Biophysical Chemistry
Göttingen, Germany.


10:00 – 10:45, Monday,13 June
Room 1, Ground Floor, Congress Centre

Read an interview with Prof. Hell

Abstract:Throughout the 20th century the resolution of optical microscopy relying on conventional lenses was limited by diffraction. We show how this limit can be radically overcome and how this change impacts various fields of science.

ECBO Plenary Speakers

Optical Imaging of Radiation Dose In Vivo
Brian Pogue, Dartmouth College, USA

Tuesday, 14 May, 14:10-14:50
Room 5, Ground Floor, Congress Centre
 
Abstract: External beam radiation from linear accelerators is routinely used as the workhorse for radiotherapy of cancer, and this delivery produces large amounts of broadband Čerenkov light in the tissue where dose is deposited.  This emission has rarely been documented, despite the widespread use of radiation therapy in cancer. 

This talk documents the first uses of this light to characterize radiation beam shapes and dose profiles in water tanks, as well as surface dose profiles delivered to tissue.  The signal is dominated by the blue end of the spectrum, which is heavily attenuated by blood, but the red and infrared wavelengths can be shown to penetrate through several centimeters of tissue, and be detected to sample the tissue features.  Monitoring of hemoglobin oxygen saturation is possible, as the absorptive attenuation of blood is well characterized, and with spectral fitting this can be estimated absolutely.  Additionally, monitoring of luminescent species in the tissue is possible, especially if they emit in the red or infrared bands. 

Monte Carlo modeling of the incident radiation and the resulting Čerenkov and luminescence can be done with GEANT4 simulations, and the data shows the sampling depth can vary from just a few millimeters up to two centimeters, based upon the wavelength chosen and the beam to fiber geometry chosen.  The utility of this signal is still unexploited to date, but should allow real-time patient dosimetry as well as correction to dose errors in treatment planning.


Fluorescence Lifetime Imaging and Metrology for Cell Biology, High Content Analysis and Clinical Diagnosis
Paul French, Imperial College London, UK

Tuesday, 14 May, 14:50-15:30
Room 5, Ground Floor, Congress Centre

Abstract: This talk will review our development and application of technology for fluorescence lifetime imaging (FLIM) and metrology applied to microscopy, high content analysis, endoscopy and tomography, aiming to translate molecular readouts across the scales.  This includes STED FLIM microscopy for multi-label super-resolution through high-speed FLIM for assays of protein interactions in automated optically sectioned multiwell plate readers to in vivo studies.  For live disease models we are developing FLIM tomography and endoscopy, including a confocal FLIM endomicroscope, wide-field FLIM endoscopes and single point fibre-optic fluorometric probes.  For clinical studies these FLIM endoscopes are complemented by multiphoton multispectral FLIM tomography.

Invited Speakers

Fully Quantitative Intrinsic Fluorescence Measurements in Turbid Media Using A Single Fiber, Arjen Amelink; Erasmus MC, Netherlands

Optical Detection Of Cancer And Precancerous Lesions of the Upper Aerodigestive Tract – Methods For Assessment Of Horizontal Extensions, Christoph Arens; University Hospital of Magdeburg, Germany

Oligonucleotide Switches And Nanomaterials For Intracellular Mrna Sensing, Francesco Baldini; IFAC-CNR, Italy

In-vivo Optical Spectroscopy In the Time-domain Beyond 1100 Nm, Ilaria Bargigia; Politecnico di Milano, Italy

Polarization Sensitive Optical Coherence Tomography For Quantitative Assessment Of Tissue Properties

In the Rat Eye In Vivo
, Bernhard Baumann; Medical University of Vienna, Austria

Three-dimensional in Vivo Blood Flow Mapping in Tumors Using Optical Frequency Domain Imaging, David Blauvelt; Harvard Medical School & Massachusetts Institute of Technology, Massachusetts General Hospital, USA

Improved Precision in Optical Tweezers Via Squeezed Light, Warwick Bowen; The University of Queensland, Australia

Towards High-throughput Fluorescence Microscopy And Quantitative Pathology for Intra-operative
Identification of Residual Disease nn Cancer Surgery,
J. Quincy Brown; Tulane University, USA

Angio-OCT as a Noninvasive Tool for Three-dimensional Vascular Network Visualization in Retinal Diseases, Danuta Bukowska; Nicolaus Copernicus University, Poland

Focusing Light in Scattering Media by Photoacoustic-guided Wavefront Shaping, Thomas Chaigne; Institut Langevin, France

Narrowband Polarisation Endoscopy with LED Illumination, Neil Clancy; Imperial College London, Imperial College London, UK

Self-interference Fluorescence Microscopy: Three Dimensional Fluorescence Imaging Without Depth Scanning, Mattijs De Groot; VU University Amsterdam, Netherlands

Multispectral Optoacoustic Tomography (msot) Imaging of Heterogeneity Within the Tumor Microenvironment, Wouter Driessen; iThera Medical, Helmholtz Zentrum München, USA

Monitoring Cell Differentiation And Infection Using Digital Holographic Microscopy, Andrew Ekpenyong; University of Cambridge, Technische Universität Dresden, UK

Optical Imaging Systems for Oral Cancer Detection and Diagnosis: A Global Perspective, Ann Gillenwater; University of Texas, USA

Quantitative Phase Contrast Optical Coherence Microscopy for Imaging Live Cell Dynamics, Branislav Grajciar; Medical University of Vienna, Austria

Monitoring Hemodynamics and Oxygenation of the Kidney in Rats Using Near-infrared Spectroscopy, Dirk Grosenick; Physikalisch-Technische Bundesanstalt, Germany

Photoacoustic Imaging of Breast Tumor Vascularization: A Comparison with MRI and Histopathology, Michelle Heijblom; University of Twente, Medisch Spectrum Twente, Netherlands

Connectomics: the Dense Reconstruction of Neuronal Circuits, Moritz Helmstaedter; Max Planck Institute of Neurobiology, Germany

Photodynamic Therapy and Photochemical Internalization in Head and Neck Cancer Management: An Overview, Colin Hopper; UCL Eastman, UK

Real-time Acousto-optic and Ultrasound Measurement for Bimodal Imaging of Small Animals, Jean-Pierre Huignard; Independent Consultant

Hyperspectral Microscopy for Chemical Mapping of Single Erythrocytes Infected by Malaria Parasites, Jeeseong Hwang; National Institute of Standards and Technology, USA

The use of Photoacoustic Tomography in Vascular Targeted Therapy to Determine Vessel Regrowth Following Treatment, Sean  Johnson; University College London, USA

Optical Resolution Imaging in the Diffusive Regime with Time-reversal Of Variance-encoded Light (trove), Benjamin Judkewitz; California Institute of Technology, USA

Gold Nanoparticle Mediated Laser Transfection for High-throughput Antisense Applications, Stefan Kalies; Laser Zentrum Hannover e.V., Germany

Combination of Optical Micromanipulation with Raman Spectroscopy for Cell Sorting, Christoph Krafft; Institute of Photonic Technology, Germany

Optical Detection of Cancer and Precancerous Lesions of the Upper Aerodigestive Tract – Methods for Assessment of Vertical Extensions, Marcel Kraft; Kantonsspital Baselland, USA

Dual Modality Breast Imaging Combining Near Infrared Spectral Tomography and Digital X-ray Tomosynthesis, Venkataramanan Krishnaswamy; Dartmouth College, USA

Structural Characterization of Human Cornea Using Polarization-resolved Shg Microscopy, Gaël Latour; Ecole Polytechnique, CNRS, INSERM U696, Univsersité Paris Sud, CNRS, France

Assessment of Brain Perfusion in Post-traumatic Brain Injury Patients by Time-resolved Optical Monitoring of Icg Inflow, Adam Liebert; Nalecz Institute of Biocybernetics and Biomedical Engineering, Poland

Towards Point-of-care Pathology for Brain Tumor Resection, Jonathan Liu; SUNY Stony Brook, USA

Sers-coded Nanoparticles for Multiplexed Molecular Endoscopy of the Esophagus, Jonathan Liu; SUNY Stony Brook, USA

Multicolor Two-photon Imaging Using Wavelength Mixing, Pierre Mahou; Laboratory for Optics and Biosciences, France

A Non-contact Time-domain Scanning Brain Imaging System: First In-vivo Results, Mikhail Mazurenka; Physikalisch Technische Bundesanstalt, Germany

The Use of Circularly Polarized Light and its Helicity for Tissue Diagnostics, Igor Meglinski; University of Otago, New Zealand

Ultrafast Force-clamp Spectroscopy to Probe Laci-dna Interactions, Carina Monico; LENS, European Laboratory for Nonlinear Spectroscopy, USA

3D Static Elastography and Shear Wave Imaging Using Full Field OCT, Amir Nahas; Langevin Institute, LLtech, France

In Living Motion:  Imaging Cellular Dynamics And Function Inside Live Three-dimensional Tissues, David Nolte; Purdue University, USA

Image-scanning Focal Modulation Microscopy for Rapid Imaging of Thick Biological Tissues, Shilpa Pant; National University of Singapore, Singapore

Real Time Quantitative Phase Imaging for Blood Screening Applications, Gabriel Popescu; Univ of Illinois at Urbana-Champaign, USA

Inverse Scattering Using Slim, Gabriel Popescu; Univ of Illinois at Urbana-Champaign, USA

Towards In Vivo Acousto-optic Measurement Using Speckle Contrast AO., Steffen Resink; University of Twente, USA

Visualizing a Cytostatic Drug and Probing Apoptosis of Cancer Cells, Herbert Schneckenburger; Hochschule Aalen, Germany

Optical Identification of Subjects at High Risk For Developing Breast Cancer, Paola Taroni; Politecnico di Milano, Italy

Physics of Temporal Pulse-to-pulse Interaction and its Consecution to Future Generation Ophthalmic Laser Systems, Nadine Tinne; Laser Zentrum Hannover e.V., Germany

Sensitivity Improvement of an All-fibre Computerized Optical Fluorescence Setup Using Dual Fibre Probes, Frederique Vanholsbeeck; University of Auckland, New Zealand

Characterization of the Bruise Healing Process  Using Pulsed Photothermal Radiometry, Luka Vidovic; Jozef Stefan Institute, USA

Characterization of Coronary Plaques with Polarization Sensitive Optical Frequency Domain Imaging, Martin Villiger; Harvard Medical School and Massachusetts General Hospital, USA

In Vivo Quantification of Hepatic Microvascular Flow Characteristics Using Laser Speckle Contrast Imaging, Jie Yan; Singapore-MIT Alliance for Research and Technology, Yong Loo Lin School of Medicine, National University of Singapore, USA